Journal:

Article Title: Analysis of Pathogen-Host Cell Interactions in Purpura Fulminans: Expression of Capsule, Type IV Pili, and PorA by Neisseria meningitidis In Vivo

doi: 10.1128/IAI.70.9.5193-5201.2002

Figure Lengend Snippet: Confocal laser scanning microscopy to detect N. meningitidis in skin biopsy samples from patients with meningococcal disease. Seven-micrometer-thick frozen sections were analyzed as sequential 1-μm-thick sections (×1,000). (A and B) A microcolony of N. meningitidis double stained with anticapsule monoclonal antibody detected with Alexa Fluor 488 (green fluorescence) and antipilin monoclonal antibody detected with Alexa Fluor 594 (red fluorescence), respectively. (C and D) A microcolony staining with antipilin monoclonal antibody (red fluorescence) and some background fluorescence but no specific staining with anticapsule monoclonal antibody (green fluorescence), respectively.

Article Snippet: The sections were then incubated for 1 h at 37°C with the biotin-coupled anticapsule meningococcal antibody (diluted 50 μg/ml in PBS containing 0.2% saponin and 10% FCS), which was then detected by incubation for 1 h at room temperature with a streptavidin-linked fluorochrome (streptavidin-conjugated Alexa Fluor 488 [Molecular Probes], diluted 10 μg/ml in PBS containing 1% FCS).

Techniques: Confocal Laser Scanning Microscopy, Staining, Fluorescence